Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preservative – buffer – anticoagulant or diluent
Patent
1991-09-05
1993-12-07
Geist, Gary
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Preservative, buffer, anticoagulant or diluent
436538, G01N 128
Patent
active
052682994
ABSTRACT:
Diluent and wash compositions are useful in a rapid and sensitive assay for detecting antibodies, and especially retroviral antibodies, in a biological specimen. The diluent composition is buffered to a pH of 6 to 10 and includes a protein or carbohydrate, a surfactant and a negatively-charged organic compound. The wash composition is buffered to a pH of 5 to 10 and includes a surfactant. These compositions can be included in a diagnostic kit. The method of this invention includes mixing the biological specimen with the diluent composition, forming an immunological complex between ligand and antibodies in the specimen and separating complexed materials from uncomplexed materials using a filtration membrane and a washing step. An enzyme labeled anti-antibody is added to form a ligand-antibody-antibody complex followed by its detection using suitable reagents.
REFERENCES:
patent: 3595755 (1971-07-01), Hartel
patent: 3764478 (1973-10-01), Bergmeyer et al.
patent: 3997470 (1976-12-01), Monte et al.
patent: 3998973 (1976-12-01), Carlson
patent: 4040785 (1977-08-01), Kim et al.
patent: 4218490 (1980-08-01), Phillips et al.
patent: 4283491 (1981-08-01), Dappen
patent: 4329448 (1982-05-01), Cox et al.
patent: 4468334 (1984-08-01), Cox et al.
patent: 4520113 (1985-05-01), Gallo et al.
patent: 4561985 (1985-12-01), Glass, Jr.
patent: 4639425 (1987-01-01), Baier
patent: 4661445 (1987-04-01), Saxinger et al.
patent: 4708818 (1987-11-01), Montagnier et al.
patent: 4725669 (1988-02-01), Essex
patent: 4808524 (1989-02-01), Snyder et al.
patent: 4812414 (1989-03-01), Warren, III et al.
patent: 4828978 (1989-05-01), Warren, III et al.
patent: 4828980 (1989-05-01), Snyder et al.
patent: 4847199 (1989-07-01), Snyder et al.
patent: 4868131 (1989-09-01), Hiratsuka
patent: 4870007 (1989-09-01), Smith-Lewis
patent: 4923680 (1990-05-01), Nelson
patent: 4935339 (1990-06-01), Zahradnik
patent: 4965191 (1990-10-01), Warren, III et al.
patent: 4997772 (1991-03-01), Sutton et al.
patent: 5017474 (1991-05-01), McClune et al.
patent: 5024935 (1991-06-01), McClune et al.
patent: 5043288 (1991-08-01), Motsenbocker
patent: 5047318 (1991-09-01), Snyder et al.
patent: 5051356 (1991-09-01), Warren, III et al.
patent: 5077198 (1991-12-01), Shih et al.
Concise Chemical and Technical Dictionary, H. Bennett, ed. Chemical Publishing Co., New York 1986, p. 1090. Ref QD5. B4.
Lambden et al., Journal of Immunological Methods, vol. 20, pp. 277-286 (1978).
Nandapalan et al., Journal of Medical Virology, vol. 14, pp. 285-294 (1984).
Frankel et al., Mol. Immunology, 16, pp. 101-106 (1979).
Morrison--Oncology & Biotechnology News,Apr. 1988.
Shih Yihsing
Smith-Lewis Margaret J.
Warren, III Harold C.
Eastman Kodak Company
Geist Gary
Tucker J. Lanny
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