Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids
Patent
1993-10-27
1995-06-13
Alexander, Lyle A.
Chemistry: analytical and immunological testing
Metal or metal containing
Present in biological fluids
436 79, 436151, 436176, 436179, G01N 3320
Patent
active
054242142
ABSTRACT:
Diluents and methodologies for determining the concentration of total calcium in a clinical sample (e.g. whole blood, sera plasma urine cerebro spinal fluid) containing protein-bound calcium are disclosed. Methodologies include the steps of: (a) admixing the sample with a diluent, the diluent including effective amounts of a pH buffer and at least two agents capable of complexing free calcium in the sample, where the agent-calcium complex association constants ("log K" in base 10) each have a range from about 1.5 to about 7.0; and (b) contacting an aliquot of the diluted sample with a calcium-specific ion selective electrode. The response of the calcium-specific ion selective electrode is an indication of the concentration of total calcium in the sample.
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Chung Suzanne C.
Houben Peter J.
Kessler James T.
Mu Xihai
Shu Frank R.
Alexander Lyle A.
Beckman Instruments Inc.
Freed Rachel Heather
Grant Arnold
May William H.
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