Dihydropyridine receptor and antibodies thereto

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530413, 530809, 43524027, 435 7021, 935104, 935108, C07K 1500, C07K 312, C12N 500

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active

049122029

ABSTRACT:
Monoclonal antibodies capable of immunoprecipitating labeled dihydropyridine receptor material from digitonin-solubilized skeletal muscle triads are disclosed. Said antibodies recognize a 170,000 dalton protein subunit of the dihydropyridine receptor.

REFERENCES:
Schmid, A. et al., "Immunochemical Analysis of Subunit Structures of 1,4-Dihydropyridine Receptors Associated with Voltage-Dependent Ca.sup.2+ Channels in Skeletal, Cardiac, and Smooth Muscles", Biochemistry 25:3492-3495, 1986.
Borsotto, M. et al., "The 1,4-Dihydropyridine Receptor Associated with the Skeletal Muscle Voltage-Dependent Ca.sup.2+ Channel", J. Biol. Chem. 260(26):14255-14263, 1985.
Curtis, B. M. et al., "Purification of the Calcium Antagonist Receptor of the Voltage-Sensitive Calcium Channel from Skeletal Muscle Transverse Tubules", Biochemistry 23(10): 2113-2118, May 8, 1984.
Mitchell, R. D. et al., "Purification of Morphologically Intact Tried Structures from Skeletal Muscle", J. Cell Biol. 96(4): 1008-16, 1983.
Goding, J. W., Monoclonal Antibodies:Principles and Practice Academic Press, Inc., Orlando, 1983, pp. 68-70.

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