Diglycosidase and gene encoding the same

Details Diglycosidase and gene encoding the same Diglycosidase and gene encoding the same

2011-08-16

2011-08-16

Nashed, Nashaat (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S254500, C435S320100, C536S023200

Reexamination Certificate

active

07998721

ABSTRACT:
A novel diglycosidase produced by a microorganism belonging to the genusPenicillium, having the following physicochemical properties:(1) action and substrate specificity: it acts on a disaccharide glycoside, releasing the disaccharide sugar and the aglycone thereof;(2) optimum pH: around 4.5;(3) pH stability: it is stable at pH 4.0 to 8.0 under the processing condition of 37° C. for 30 minutes, and retains its 80% or more of the activity even after processing at pH 4.0 or lower;(4) optimum temperature: around 60° C. in a sodium acetate-acetic acid buffer solution (pH 5.5);(5) thermal stability: it is stable at 50° C. or lower in a sodium acetate-acetic acid buffer solution (pH 5.5) and retains 45% of the activity even after processing at 60° C. for 40 minutes;(6) molecular weight: 40,000±5,000 Da based on SDS-PAGE measurement; and(7) isoelectric point (pI): about 4.3.

REFERENCES:
patent: 7109014 (2006-09-01), Yamamoto et al.
patent: 7118895 (2006-10-01), Tsuruhami et al.
patent: 2003/0194469 (2003-10-01), Tsuruhami et al.
patent: 2005/0208177 (2005-09-01), Tsuruhami et al.
patent: 08-140675 (1996-06-01), None
patent: WO 00/18931 (2000-04-01), None
patent: WO 03/087290 (2003-10-01), None
Tsuruhami et al., “Isolation and Characterization of a β-Primeberosidase-Like Enzyme fromPenicillium multicolor” Biosci. Biotechnol. Biochem, 70(3): 691-698 (2006).

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