Digitalis antibodies, process for the preparation thereof and th

Details Digitalis antibodies, process for the preparation thereof and th Digitalis antibodies, process for the preparation thereof and th

1983-12-19

1988-05-03

Wiseman, Thomas G.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530387, 530389, 530391, 530413, 530415, 530416, 424 85, 514 6, 435 7, 435 68, 435 69, 436538, 436541, C07K 1300, C12Q 100, C12P 2100

Patent

active

047421592

ABSTRACT:
The present invention provides a process for the preparation of digitalis antibodies in which appropriate mammals are immunized with a digoxin bound to protein, the animal serum is obtained, the immune globulin-containing protein fraction is separated in known manner, the immunologically-active globulins are adsorbed on an immunologically-active column and separated from the other proteins, the antibodies are again eluted from the column and the Fab fractions are split with papain and purified, wherein, as immunologically-active adsorbent, there is used an inorganic matrix of large surface area to which digitoxin aldehyde is bound via a spacer which cannot be split with papain and the splitting off of the Fab fraction from the antibodies is carried out on the matrix.
The present invention also provides digitalis antibodies obtained by this process, which antibodies can be used for the therapy of digitalis intoxications and for preparing immunological reagents for the determination of digitalis glycosides.

REFERENCES:
patent: 3790475 (1974-02-01), Eaton
patent: 4081245 (1978-03-01), Polito
patent: 4081246 (1978-03-01), Polito
Sugura et al, Chemical Abstracts, vol. 94, No. 135598n, "New Immunochemical Conjuguko", 1981, p. 396.
Iskikaur et al, Chemical Abstract, vol. 89, No. 19774g, 1978, "Antigen and Antibody Determinator", p. 301.
Smith et al, New England J. Med., vol. 307, No. 22, pp. 1357-1362, Nov. 1982, "Treatment of Life-Threatening Digitalis Intoxication . . . ".
Smith et al, New England J. Med., vol. 294, No. 15, 1976, pp. 797-800, "Reversal of Advanced Intoxication with Fab Fragments of Digoxin-Specific Antibodies".

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