Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Protein or peptide standard or control
Reexamination Certificate
2008-10-08
2011-11-08
Drodge, Joseph (Department: 1778)
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Protein or peptide standard or control
C204S451000, C210S638000, C210S639000, C422S082050, C422S504000, C435S007100, C435S287300, C435S288700, C436S086000, C436S164000, C436S172000, C436S178000, C436S180000
Reexamination Certificate
active
08053239
ABSTRACT:
A method for extracting proteins from heterogeneous fluids by precipitation using microfluidics. The method uses an automated protocol for precipitation of proteins onto surfaces, rinsing the precipitates to remove impurities, and resolubilization in buffer for further analysis. The method is compatible with proteins representing a range of different physicochemical properties, as well as with complex mixtures such as fetal bovine serum and cell lysate. In all cases, the quantitative performance (measured using a fluorescent assay for % recovery) was comparable to that of conventional techniques, which are manual and require more time.
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Jebrail Mais J.
Wheeler Aaron R.
Drodge Joseph
Hill & Schumacher
Schumacher Lynn C.
The Governing Council of the University of Toronto
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