Differentiation of nucleic acid segments on the basis of nucleot

Details Differentiation of nucleic acid segments on the basis of nucleot Differentiation of nucleic acid segments on the basis of nucleot

1988-11-15

1989-11-07

Lilling, Herbert J.

Bleaching and dyeing; fluid treatment and chemical modification

Treatment of hides, skins, feathers and animal tissues

Tanning

435 91, 935 1, 935 2, 935 3, 935 8, 935 17, 935 18, 935 76, 935 77, C12Q 168

Patent

active

048792144

ABSTRACT:
A process for distinguishing nucleic acid segments on the basis of nucleotide differences, thereby providing a rapid, convenient means for detecting mutation, is disclosed. The present process comprises synthesizing separately complementary nucleic acid strands on each of at least two target nucleic acid templates using a nucleic acid polymerase and nucleoside triphosphate substrates, wherein at least one of the natural nucleoside triphosphate substrates is replaced with a mobility-shifting analog; denaturing the synthesized strands from the templates if necessary; and comparing the mobility of the separately synthesized strands through a size-fractionation medium. A difference in mobility indicates that the target nucleic acid templates contain different numbers of total nucleotides and/or different numbers of nucleotides complementary to the mobility-shifting analogs. Thus, the process of the present invention can be used to distinguish homologous DNAs or RNAs differing by nucleotide substitutions that affect the number of mobility-shifting analog residues incorporated into synthesized strands, as well as to distinguish differences due to nucleotide insertion or deletion.

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