Differentiation of flow cytometry pulses and applications

Optics: measuring and testing – Velocity or velocity/height measuring – With light detector

Reexamination Certificate

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Reexamination Certificate

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07847923

ABSTRACT:
A method of analyzing pulses from a flow cytometer in which particles in a fluid pass through an excitation volume of an electromagnetic radiation and interact with the electromagnetic radiation to generate signals in the form of pulses includes generating a time-dependent pulse indicative of the characteristics of one or more particles passing through the excitation volume of the electromagnetic radiation, determining a measurement window by selecting a portion of the pulse with a starting point and an ending point above a predetermined value, and calculating a first derivative of the pulse with respect to time over the measurement window.

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