Differential lymphoid-myeloid determination of leukocytes in who

Details Differential lymphoid-myeloid determination of leukocytes in who Differential lymphoid-myeloid determination of leukocytes in who

1979-11-23

1981-09-01

Turk, Arnold

Chemistry: physical processes

Physical processes

Crystallization

23913, 252408, G01N 3348, G01N 3372

Patent

active

042869633

ABSTRACT:
This invention relates to a lytic diluent and a method for rapid lysing of red blood cells in whole blood for a differential determination of lymphoid and myeloid populations of leukocytes, and measurement of hemoglobin, particularly for use in automatic particle counting systems.
The diluent is a mixture of at least one quaternary ammonium salt having surface active properties, and an additive in suitable proportions, buffered to an acid pH of 3.5 to 5.0. The additive includes 2-phenoxyethanol and other short chain alkanols substituted by one phenyl or phenoxy, as well as certain polyhydroxy compounds.
When the reagent is employed in lower concentrations, an alkaline reagent comprising a quaternary ammonium salt and an alkali metal cyanide, buffered to an alkaline pH, may be added to complete the chromagen formation.

REFERENCES:
patent: 3874852 (1975-04-01), Hamill
patent: 3962125 (1976-06-01), Armstrong
patent: 3964865 (1976-06-01), Das
patent: 4102810 (1978-07-01), Armstrong
patent: 4185964 (1980-01-01), Lancaster

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