Differential hemolysis of a whole blood sample

Details Differential hemolysis of a whole blood sample Differential hemolysis of a whole blood sample

2011-05-31

2011-05-31

Wallenhorst, Maureen M (Department: 1777)

Chemistry: analytical and immunological testing

Biological cellular material tested

C436S161000, C436S173000, C436S174000, C210S656000, C250S282000

Reexamination Certificate

active

07951598

ABSTRACT:
The invention relates to a method for differentially hemolyzing whole blood. It discloses methods for detecting an analyte in a liquid sample known or suspected to contain red blood cells and suspected or known to contain eukaryotic cells, the method including the steps of processing the liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may contain both erythrocytes and nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

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