Differential diagnostic assay for brucellosis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 75, 435 79, 435 793, 43524027, 435975, 436518, 5303884, 530810, 530825, G01N 33569, G01N 33577, C07K 1528, C12N 500

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active

051908603

ABSTRACT:
An immunoassay procedure is provided which exhibits increased specificity over current procedures. The procedure allows the differentiation of animals infected with Brucella abortus from animals vaccinated with Brucella abortus Strain 19. The invention employs unique monospecific monoclonal antibodies having particular affinity, specificity and binding characteristics directed to a B. abortus lipopolysaccharide antigen. The invention also concerns continuous hybrid cell lines for producing the unique monoclonal antibodies.

REFERENCES:
patent: 4228237 (1980-10-01), Hevey et al.
patent: 5006463 (1991-04-01), Cherwonogrodzky et al.
P. Tijssen, Practice and Theory of Enzyme Immunoassays, pp. 21-31 (1985).

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