Differential assay of creatine phosphokinase isoenzymes

Details Differential assay of creatine phosphokinase isoenzymes Differential assay of creatine phosphokinase isoenzymes

1975-09-26

1976-11-30

Monacell, A. Louis

Textiles: fiber preparation

C12K 104

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039947831

ABSTRACT:
A process for the determination of creatine phosphokinase MB-isoenzyme (CPK-MB) level in serum in which CPK is determined before and after MB-activation with 1,4-dimercapto-2,3-dihydroxy butane or 2-mercaptoethanol, preferably in an assay system using the ATP/ADP conversion. The difference in the two values found is due to the CPK-MB present. The invention is of particular utility in the diagnosis and treatment of myocardial damage.

REFERENCES:
patent: 3540984 (1970-11-01), Deutsch
Warren, "Activation of Serum Creative Kinase by Dithiothreitol", Clinical Chem., vol. 18, No. 5, 1972, pp. 473-475.
Roe et al., "Combined Isoenzyme Analysis in the Diagnosis of Myocardial Injury: Application of Electrophoretic Methods for the Detection and Quantitative of the Creative Phosphokinase MB Isoenzyme", J. of Laboratory and Clinical Medicine, 80, (1972), pp. 577-590.

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