Difference gel electrophoresis using matched multiple dyes

Details Difference gel electrophoresis using matched multiple dyes Difference gel electrophoresis using matched multiple dyes

1997-10-10

2000-03-28

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

204459, 204461, 435810, 436 63, 436 86, 436172, 436800, C12Q 100, G01N 3368

Patent

active

060430257

ABSTRACT:
A process and a kit are provided for detecting differences in two or more samples of protein. Protein extracts are prepared, for example, from each of a different group of cell samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and electrophoresed together. The gel is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.

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