Difference detection methods using matched multiple dyes

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S004000, C436S063000, C436S800000, C530S344000, C530S412000, C424S001570

Reexamination Certificate

active

07598047

ABSTRACT:
A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides. Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.

REFERENCES:
patent: 4774339 (1988-09-01), Haugland et al.
patent: 4855225 (1989-08-01), Fung et al.
patent: 4874492 (1989-10-01), Mackay
patent: 5242796 (1993-09-01), Prober et al.
patent: 5248782 (1993-09-01), Haugland et al.
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5274113 (1993-12-01), Kang et al.
patent: 5296599 (1994-03-01), Cohen et al.
patent: 5307148 (1994-04-01), Kambara et al.
patent: 5312921 (1994-05-01), Glazer et al.
patent: 5338854 (1994-08-01), Kang et al.
patent: 5364764 (1994-11-01), Haugland et al.
patent: 5512486 (1996-04-01), Giese et al.
patent: 5569587 (1996-10-01), Waggoner
patent: 5627027 (1997-05-01), Waggoner
patent: 5654419 (1997-08-01), Mathies et al.
patent: 6043025 (2000-03-01), Minden et al.
patent: 6048982 (2000-04-01), Waggoner
patent: 6127134 (2000-10-01), Minden et al.
patent: 6225050 (2001-05-01), Waggoner
patent: 6426190 (2002-07-01), Minden et al.
patent: 2002/0177122 (2002-11-01), Minden et al.
patent: 709733 (1999-12-01), None
patent: 0 272 007 (1988-06-01), None
patent: 0 504 943 (1992-09-01), None
patent: 5322770 (1993-12-01), None
patent: 5322770 (1994-09-01), None
patent: 5322771 (1994-09-01), None
patent: WO 92/14747 (1992-03-01), None
patent: WO 94/17397 (1994-08-01), None
patent: WO 96/33406 (1996-10-01), None
patent: WO 00/66792 (2000-11-01), None
Goldman et al. (European Journal of Biochem., 131 pp. 473-480, 1983).
Potter (Electrophoresis, 1990, vol. 11, pp. 415-419).
Toda et al. (Journal of Chromatography A, 698, 1995, pp. 41-54).
Anderson et al. (Clinical Chemistry, 1981, vol. 27, No. 11, pp. 1807-1820).
Sargent P.B. (NeuroImage, 1994, vol. 1, No. 4, pp. 288-295).
Luby-Phelps et al. (Biophysical Journal, vol. 65, pp. 236-242, Jul. 1993).
Amersham Life Science Products Catalogue (1991) p. 61, Illinois, USA “Rainbow Protein Molecular Weight Markers.”
Amersham Pharmacia Biotech “BioDirectory '99” catalogue, section entitled “Chromatographic Columns and Media,” pp. 502-588 (1999).
Opiteck et al., “Comprehensive On-Line LC/LC/MS of Proteins,” Anal. Chem., vol. 69, pp. 1518-1524 (1997).
Opiteck et al., “Comprehensive Two-Dimensional High Performance Liquid Chromatography for the Isolation of Overexpressed Proteins and Proteome Mapping,” Anal. Biochem., vol. 258, pp. 349-361 (1998).
Jensen et al., “Probing Proteomes Using Capillary Isoelectric Focussing-Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry,” Anal. Chem. vol. 71, pp. 2076-2084 (1999).
Wilchek, M. and Bayer, E.A., “Labelling Glycoconjugates with Hydrazide Reagents,” Methods in Enzymology, vol. 138, pp. 429-442 (1987).
Mujumdar, R.B. et al., “Cyanine Dye Labelling Reagents Containing Isothiocyanate Groups,” Cytometry, vol. 10, pp. 11-19 (1989).
Chasman, D.I. et al., “Activation of Yeast Polymerase II Transcription by Herpesvirus VP16 and GAL4 Derivatives In Vitro,” Molecular and Cellular Biology, vol. 9, No. 11, pp. 4746-4789 (1989).
Letter to the Editor, “HBL-100 Cells Do Not Secrete Casein and Lack Prolactin and Estradiol Receptors,” In Vitro Cell Dev. Biol., vol. 26, pp. 933-935, (1990).
Opiteck et al., “Two Dimensional SEC/RPLC Coupled to Mass Spectrometry for the Analysis of Peptides,” Anal. Chem. vol. 69, pp. 2283-2291, (1997).
Lasfarques et al. “Isolation of Two Human Tumor Epithelial Cell Lines from Soild Breast Carcinomas,” J. Natl. Cancer Inst., vol. 61, No. 4, pp. 967-973, (1978).
Bhargava et al., “Phosphorylation of Parathyroid Secretory Protein,” Proc. Natl. Acad. Sci., vol. 80, pp. 878-881, (1983).
Wayne F. Patton, “A Thousand Points of Light: The Application of Fluorescence Detection Technologies to Two-dimensional Gel Electrophoresis and Proteomics,” Electrophoresis, vol. 21, pp. 1123-1144 (2000).
Juan F. Santaren et al., “Identification ofDrosophilaWing Imaginal Disc Proteins by Two Dimensional Gel Analysis and Microsequencing,” Experimental Cell Research 206, pp. 220-226, (1993).
James I. Garrels, “Two-Dimensional Gel Electrophoresis and Computer Analysis of Proteins Synthesized by Clonal Cell Lines,” Journal of Biological Chemistry, vol. 254, No. 16, pp. 7961-7973, (1979).
Peter Jackson et al., “Rapid Imaging, Using a Cooled Charged-Coupled-Device of Fluorescent Two-dimensional Polyacrylamide Gels Produced by Labeling Proteins in the First Dimensional Isoelectric Focusing Gel with the Fluorophore 2-methoxy-2,4-diphenyl-3 (2H) furanone,” Electrophoresis, vol. 9, pp. 330-339, (1988).
Patrick H. O'Farrell, High Resolution Two Dimensional Electrophoresis of Proteins, Journal of Biological Chemistry, vol. 250, No. 10, pp. 4007-4021, (1975).
Valerie E. Urwin et al., “A Multiple High Resolution Mini-Two Dimensional Polyacrylamide Gel Electrophoresis System: Imaging Two-Dimensional Gels using a Cooled Charged-Coupled Device After Staining with Silver of Labeling with Fluorophore,” Analytical Biochemistry, vol. 195, pp. 30-37, (1991).
Richard P. Haughland, “Handbook of Fluorescent Probes and Research Chemicals,” 5th Edition, pp. 203-214, 1992-1994.
R.F. Steiner (Ed), “Excited States of Biopolymers,” Plenum Press, New York, pp. 29-58, (1983).
U.K. Laemmli, “Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4,” Nature vol. 227, pp. 680-685, (1970).
Biological Detection Systems Inc., “FluoroLink-AbtmCy5tmLabeling Kit” 1995 Cat. No. A35000.
Biological Detection Systems Inc., “FluoroLink-AbtmCy3.5tmLabeling Kit”.
Biological Detection Systems Inc., “FluoroLink-AbtmCy3tmLabeling Kit”.
Biological Detection Systems Inc., “FluoroLink-AbtmCy2tmLabeling Kit”.
Biological Detection Systems, Inc. “Fluorolink Labeling Reagents” (1999).
Molecular Probes Inc., “Conjugation with Amine-Reactive Probes,” Product Information Sheet. (1999).
Molecular Probes Inc., “Conjugation with Thiol-Reactived Probes,” Product Information Sheet.
Sothwick P.L. et al., “Cyanine Dye Labeling Reagents-Carboxymethylindocyanine Succinimidyl Esters”, Cytometry, vol. 11, pp. 418-430, (1990).
Lansing Taylor D. et al., “The New Vision of Light Microscopy. Lasers, Electronics Cameras and Digital Image analysis Combined with the Most Venerable Instrument of the Life Sciences to Create New Ways of Seeing Living Cells,” American Scientist, New Haven, CT, vol. 80, No. 4, pp. 322-325, (1992).
L.M. Smith et al., “Florescence Detection in Automated DNA Sequence Analysis,” Nature vol. 321. pp. 674-678, (1986).
Unlu et al., “Difference Gel Electrophoresis: A Single Gel Method for Detecting Changes in Protein Extracts,” Electrophoresis, vol. 18, pp. 2071-2077, (1997).
Chang, J.Y., et al., “A New Method for the Selective Isolation of Cystein-containing Peptides. Specific Labelling of the Thiol Group with a Hydrophobic Chromaphore,” Biochem. J., vo

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