Diequatorially bound .beta.-1, 4 polyuronates and use of same fo

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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514 8, 514929, 514 54, 514908, 536 2, 536 3, 536 20, 536127, 536114, 536123, 435 891, 424 852, A61K 3012, C12R 191, C12R 500, C12R 502

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051698404

ABSTRACT:
The present invention is directed to a material and method for the stimulation of the production of cytokines. Several polysaccharides, including polymers of different size of .sym.1-4 linked D-mannuronic acid (poly-M and M-blocks), chitosan and cellulose oxidized in C-6 position C60XY) induce human monocytes to produce the cytokines. Preferably, the molecular weights of poly-M and chitosan are above 50,000 and 20,000 respectively. Pretreatment of the monocytes with IFN-.gamma. increases the cytokine production from monocytes stimulated with all polysaccharides tested. The subject polysaccharides worked in vivo and in vitro.
The present invention has therapeutic utility as vaccine adjuvants and components. Therapeutic compositions comprising biologically active quantities of the compositions of the present invention may be employed to potentiate antibody production in response to vaccine antigens. Anti-tumor, anti-bacteriological, anti-fungal and anti-viral effects may be expected.

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Painter et al.; Carbohydrates Research, 140 (1985) 61-68; "New Gluco-noglucans Obtained by Oxidation of Amylose at Position 6".
Skjak-Braek et al.; Carbohydrate Research, 154 (1986) 239-250; "Monomer Sequence & Acetylation Pattern In Some Bacterial Alignates".
Browning et al.; Journal of Immunology; 138(9), 2857-63; "Interferon Blocks Interleukin 1-induced Prostaglandin Release From Human Peripheral Monocytes".
Holter et al.; Journal of Immunology; 138(9), 2917(22); "Expression of Functional IL-2 Receptors by Lipolysaccharides and Interferron-.gamma.Stdm Human Monocytes".

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