Dielectrophoretic separation of stained cells

Details Dielectrophoretic separation of stained cells Dielectrophoretic separation of stained cells

2006-12-26

2006-12-26

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

C435S366000, C435S372000, C435S173100, C435S173400, C435S173500, C204S450000, C204S547000

Reexamination Certificate

active

07153648

ABSTRACT:
This invention relates generally to the field of cell separation or isolation. In particular, the invention provides a method for separating cells, which method comprises: a) selectively staining cells to be separated with a dye so that there is a sufficient difference in a separable property of differentially stained cells; and b) separating said differentially stained cells via said separable property. Preferably, the separable property is dielectrophoretic property of the differentially stained cells and the differentially stained cells are separated or isolated via dielectrophoresis. Methods for separating various types of cells in blood samples are also provided. Centrifuge tubes useful in density gradient centrifugation and dielectrophoresis isolation devices useful for separating or isolating various types of cells are further provided.

REFERENCES:
patent: 3975156 (1976-08-01), Kraft et al.
patent: 4326934 (1982-04-01), Pohl
patent: 5641628 (1997-06-01), Bianchi
patent: 5653859 (1997-08-01), Parton et al.
patent: 5814200 (1998-09-01), Pethig et al.
patent: 5866071 (1999-02-01), Leu
patent: 5888370 (1999-03-01), Becker et al.
patent: 5948278 (1999-09-01), Sammons et al.
patent: 6071394 (2000-06-01), Cheng et al.
patent: 6596143 (2003-07-01), Wang et al.
patent: WO-99/62622 (1999-12-01), None
patent: WO 02/16647 (2002-02-01), None
patent: WO-02/27909 (2002-04-01), None
patent: WO 02/28523 (2002-04-01), None
patent: WO 02/29400 (2002-04-01), None
Shafer, J.A., “Hematology,” 1995, Churchill Livingstone, Hoffman et al (Eds.), 2nd Ed., pp. 2202-2203.
Miale, J.B., “Laboratory Medicine: Hematology,” 1972, C.V. Mosby Company, 4th Ed., pp. 1208-1209.
Cheng et al, “Isolation of cultured cervical carcinoma cells mixed with peripheral blood cells on a bioelectronic chip,” 1998, Anal. Chem., vol. 70, No. 11, pp. 2321-2326.
Merriam-Webster Online Dictionary, 2004, www.m-w.com, accessed Jul. 28, 2004.
Talary et al, “Electromanipulation and separation of cells using travelling electric fields,” J. Phys. D: Appl. Phys, 1996, vol. 29, pp. 2198-2203.
Becker et al., Proc. Natl. Acad. Sci. USA (1995) 29:860-864.
Bianchi et al., Proc. Natl. Acad. Sci. USA (1990) 86:3279-3283.
Cheng et al., Nature Biotechnology (1998) 16(6):541-546.
Cheung et al., Nature Genetics (1996) 14:264-268.
Fuhr et al., Sensors and Materials (1995) 7:131-146.
Holzgreve et al., Journal of Reproductive Medicine (1992) 37(5):410-418.
Huang et al., Biochim. Biophys. Acta (1996) 1282-76-84.
Huang et al., Biophysical Journal (1997) 73:1118-1129.
Huang et al., J. Phys. D. Appl. Phys. (1993) 26:1528-1535.
Hughes et al., J. Phys. Appl. Phys. (1997) 29:474-482.
Muller, Biosensors & Bioelectronics (1999) 14:247-256.
Pethig, Critical Reviews in Biotechnology (1996) 16(4):331-348.
Simpson and Elias, Prenatal Diagnosis (1994) 14:1229-1242.
Wang et al., Biochim. Biophys. Acta (1995) 1243:185-194.
Wang et al., Biophys. J. (1997) 72:1887-1899.
Wang et al., J Phys. (1994) 27:1571-1574.
Williamson and Bob, Nature Genetics (1996) 14:239-240.
Chan et al., Biochimica et Biophysica Acta (2000) 1500(3):313-322.
Kuo and Guo, Obstetrics and Gynecology (1999) 94(3):464-468.
Mavrou et al., In Vivo (1998) 12(2):195-200.
Shafer et al., Hematology: Basic Principles and Practice, 2nd Edition, Churchill Livingstone, Edinburgh, Scotland (1995) pp. 2202-2209.
Supplementary Partial European Search Report for EP 02 72 8540, mailed on Apr. 4, 2005, 7 pages.

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