Dideoxy dye terminators

Details Dideoxy dye terminators Dideoxy dye terminators

2005-09-27

2005-09-27

Fredman, Jeffrey (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C435S006120, C435S091200

Reexamination Certificate

active

06949635

ABSTRACT:
A kit for DNA sequencing comprising a first, second, third and fourth dye terminator molecules, each of the dye terminator molecules comprising a dye molecule, a linker of at least 10 atoms in length and either ddATP, ddCTP, ddGTP or ddTTP as a mono or tri-phosphate and a thermostable DNA polymerase.

REFERENCES:
patent: 5187085 (1993-02-01), Lee
patent: 5242796 (1993-09-01), Prober et al.
patent: 5306618 (1994-04-01), Prober et al.
patent: 5332666 (1994-07-01), Prober et al.
patent: 5558991 (1996-09-01), Trainor
patent: 5614365 (1997-03-01), Tabor et al.
patent: 5795782 (1998-08-01), Abramson et al.
patent: 91/05060 (1991-04-01), None
patent: WO98/58942 (1998-12-01), None
patent: WO 99/40223 (1999-08-01), None
Evangelista et al, “Characterization of fluorescent nucleoside triphosphates by capillary electrophoresis with laser induced fluorescence detection: action of alkaline phosphatase and DNA polymerase”, Anal. Biochem. 235:89-97.
Haralambidis et al, “Preparation of base-modified nucleosides suitable for non-radioactive label attachment and their incorporation into synthetic oligodeoxyribonucleotides” , Nucleic Acids Research 15(12):4857-4876.
Fuller, C. W. et al., “Variation in band intensities in DNA sequencing,”Comments16(3):1-8 (1989).
Lee, L. G. et al., “DNA sequencing with dye-labeled terminators and T7 DNA polymerases: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments,”Nucl. Acids Res.20(10):2471-2483 (1992).
Parker, L. T. et al., “AmpliTaq® DNA polymerase, FS dye-terminator sequencing: analysis of peak height patterns,”BioTechniques21(4):694-699 (1996).
Parker, L. T. et al., “Peak height variations in automated sequencing of PCR products using Taq dye-terminator chemistry,”BioTechniques19(1):116-121 (1995).
Prober, J. M. et al., “A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides,”Science238:336-341 (1987).
Rosenblum, B. B. et al., “New dye-labeled terminators for improved DNA sequencing patterns,”Nucl. Acids Res.25(22):4500-4504 (1997).
P.B. Vander Horn, “Thermo Sequenase™ DNA Polymerase andT. acidophilumPyrophosphatase: New Thermostable Enzymes for DNA Sequencing”, BioTechnics, vol. 22, No. 4 (1997) pp. 758-765.

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