Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-09-07
1996-10-15
Rollins, John W.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 435814, 435815, C12N 958, C12N 948
Patent
active
055653499
ABSTRACT:
A dipeptidylaminopeptidase (dDAP enzyme) and a method for isolating it from the culture medium of the cellular slime mold, Dictyostelium discoideum have been presented. The isolated dDAP enzyme has a pH optimum of about 3.5 and a mass of about 225 kDA. The dDAP enzyme has an activity which is somewhat similar to both DAP-I and DAP-III from this organism. Methods for using the dDAP enzyme to remove dipeptides from the N-terminus of recombinantly produced precursor proteins or peptides are also presented.
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Atkinson Paul R.
Hilton Matthew D.
Lambooy Peter K.
Eli Lilly and Company
Maciak Ronald S.
Norman Douglas K.
Rollins John W.
Weber Jon P.
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