Dictyostelid expression vector and method for expressing a desir

Details Dictyostelid expression vector and method for expressing a desir Dictyostelid expression vector and method for expressing a desir

1995-05-26

1998-04-07

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 693, 435171, 4351723, 435243, 43525411, 4353201, 536 231, 536 241, 935 48, C12P 2106, C12N 1580, C12N 115, C07H 2104

Patent

active

057363587

ABSTRACT:
The invention relates to recombinant DNA molecules comprising a Dictyostelium discoideum homologous promoter region, a heterologous DNA sequence with a Dictyostelium discoideum homologous peptide sequence capable of functioning as a leader peptide sequence positioned upstream thereof and in proper reading frame therewith, and a Dictyostelium discoideum homologous termination region, the heterologous DNA sequence encoding the desired functional polypeptide or intermediate thereof, said DNA sequence being positioned downstream from the promoter region and said termination region being positioned downstream from the DNA sequence. The invention also provides recombinant dictyostelid hosts as well as methods for preparing recombinant DNA molecules of the invention and for expressing recombinant polypeptides encoded by said recombinant DNA molecules in dictyostelid hosts. The invention in particular relates to the expression of the CS protein of Plasmodium falciparum.

REFERENCES:
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