Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Patent
1997-06-11
1998-09-22
Alexander, Lyle A.
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
436 86, 436175, 436 63, G01N 3348, G01N 33487
Patent
active
058113070
ABSTRACT:
The novel dicationic dye diimide (DAPER) is prepared by reacting 3,4,9,10-perylenetetracarboxylic dianhydride with N,N-dimethylaminopropylamine. The dye DAPER is particularly useful in a rapid, sensitive method for the precipitation, recovery and quantitation of DNA from solutions containing DNA, which method involves the steps of (a) adding the dye DAPER to a solution containing DNA and allowing the resulting mixture to equilibrate; (b) collecting the resulting precipitate; (c) removing weakly bound DAPER from the precipitate; (d) extracting DAPER therefrom by the addition of a solvent therefor; and (e) determining the amount of DNA precipitated by measuring either the absorbance or fluorescence of DAPER in the solvent extract from step (d).
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Zhi-Ren Liu and R.L. Rill, Chemical Abstracts, vol. 124 No. 19, issued May dicationic perylene dye for rapid precipitation and quantitation of trade amounds of DNA Anal. Biochem. vol. 236, No. 1 pp. 139-145. (Eng.).
Liu Zhi-Ren
Rill Randolph L.
Alexander Lyle A.
Florida State University
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