Dicarboxylic acid transport genes

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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536 27, 4352522, 4352523, 4353201, 935 42, 935 59, C12N 1511, C12N 1563, C12N 121, C07H 1512

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active

050772090

ABSTRACT:
The invention relates to DNA fragments encoding genes which complement dicarboxylic acid transport mutants of Rhizobium species to plasmids encoding such DNA sequences and to bacterial hosts carrying the said plasmids. The invention further relates to a method of increasing dicarboxylic acid transport in Rhizobium species and to a method of increasing the nitrogen fixing ability of plants by infecting or inoculating the plants with bacterial hosts containing the above described DNA fragments.

REFERENCES:
Ronson, Astwood & Dowine, Modecular Cloning and Genetic Organization of C.sub.4 -Dicarboxylate Transport Genes from Rhizobium leguminosarum (1984), J. Bacteriol. 160, 903-909.
Ronson, Lyttleton & Robertson, C.sub.4 -Dicarboxylate Transport Mutants of Rhizobium Trifolii Form Ineffective Nodules on Trifolium Repens (1981), PNAS (U.S.A.), 78, 4284-4288.
Finan, Wood & Jordan, Symbiotic Properties of C.sub.4 dct Mutants, J. Bact. 154:1403-1413 (1983).
Ditta, Stanfield, Corbin & Helsinki, Broad Host-Range DNA Cloning System, PNAS 77:7347-7351 (1980).

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