Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Heterocyclic carbon compounds containing a hetero ring...
Reexamination Certificate
2002-01-09
2004-06-29
Raymond, Richard L. (Department: 1624)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Heterocyclic carbon compounds containing a hetero ring...
C514S253100, C514S254020, C544S121000, C544S304000, C544S367000
Reexamination Certificate
active
06756374
ABSTRACT:
FIELD OF THE INVENTION
The present invention is directed to novel diaminothiazoles of formula
These compounds inhibit cyclin-dependent kinase 4 (Cdk4) and are selective against Cdk2 and Cdk1. These compounds and their pharmaceutically acceptable salts and esters have antiproliferative activity and are useful in the treatment or control of cancer, in particular solid tumors. This invention is also directed to pharmaceutical compositions containing such compounds and to methods of treating or controlling cancer, most particularly the treatment or control of breast, lung and colon and prostate tumors. Finally, this invention is also directed to novel intermediate compounds useful in the preparation of a compound of formula I.
BACKGROUND OF THE INVENTION
Uncontrolled cell proliferation is the hallmark of cancer. Cancerous tumor cells typically have some form of damage to the genes that directly or indirectly regulate the cell-division cycle.
The progression of cells through the various phases of the cell cycle is regulated by a series of multienzyme complexes consisting of a regulatory protein, a cyclin, and a kinase. These kinases are called cyclin-dependent kinases (Cdks). The Cdks are expressed throughout the cell cycle, while the levels of the cyclins vary depending on the stage of the cell cycle.
The transition from G
1
phase into S phase is regulated by the complex of Cdk4 with cyclin D. This complex phosphorylates the tumor supressor protein Retinoblastoma (pRb), releasing the transcription factor E2F and allowing the expression of genes required in S phase (Nevins, J. R.
Science
1992, 258, 424-429). Blocking the activity of the Cdk4/cyclin D complex arrests the cell cycle in G
1
phase. For example, the proteins of the INK4 family, including p16
INK4a
, which block the kinase activity of the Cdk4/cyclin D complex, cause arrest in G
1
(Sherr, C. J.
Science
1996, 274, 1672-1677).
Recent experiments show that the complex of Cdk4 with cyclin D3 also plays a role in cell cycle progression through G
2
phase. Inhibition of this complex, either by p16 or using a dominant negative Cdk4, results in arrest in G
2
phase in cells that do not express pRb (Gabrielli B. G. et al.
J. Biol. Chem.
1999, 274, 13961-13969).
Numerous defects in the pRb pathway have been shown to be involved in various cancers. For example, overexpression of Cdk4 has been observed in cases of hereditary melanoma (Webster, K. R.
Exp. Opin. Invest. Drugs
1998, 7, 865-887); cyclin D is overexpressed in many human cancers (Sherr, C. J.
Science
1996, 274, 1672-1677); p16 is mutated or deleted in many tumors (Webster, K. R.
Exp. Opin. Invest Drugs
1998, 7, 865-887); and pRb function is lost through mutation or deletion in many human cancers (Weinberg, R. A.
Cell
1995, 81, 323-330). Defects in this pathway have also been shown to have an effect on prognosis. For example, loss of p16 is correlated with poor prognosis in non-small-cell lung carcinoma (NSCLC) and malignant melanoma (Tsihlias, J. et al.
Annu. Rev. Med.
1999, 50, 401-423).
Because of the involvement of the Cdk4/cyclin D/pRb pathway in human cancer through its role in regulating progression of the cell cycle from G
1
to S phase, and the potential therapeutic benefit from modulating this pathway, there has been considerable interest in agents that inhibit or promote elements of this pathway. For example, effects on cancer cells have been shown using antibodies, antisense oligonucleotides and overexpression or addition of proteins involved in the pathway. See, e.g., Lukas, J. et al.
Nature
1995, 79, 573-582; Nevins, J. R.
Science
1992, 258, 424-429; Lim, I. K. et al.
Molecular Carcinogenesis
1998, 23, 25-35; Tam, S. W. et al.
Oncogene
1994, 9, 2663-2674; Driscoll, B. et al.
Am. J. Physiol.
1997, 273 (
Lung Cell. Mol. Physiol.
), L941-L949; and Sang, J. et al.
Chin. Sci. Bull.
1999, 44, 541-544). There is thus an extensive body of literature validating the use of compounds inhibiting targets in the Cdk4 pathway as anti-proliferative therapeutic agents.
It is thus desirable to identify chemical inhibitors of Cdk4 kinase activity. It is particularly desirable to identify small molecule compounds that may be readily synthesized and are effective in inhibiting Cdk4 or Cdk4/cyclin complexes, for treating one or more types of tumors.
There are several examples of small molecule inhibitors of the cyclin-dependent kinases, including Cdk4 (Rosania, G. R. et al.
Exp. Opin. Ther. Patents
2000, 10, 215-230). Several of these compounds inhibit multiple targets.
For example, Flavopiridol (Aventis)
is in Phase II clinical trials for lymphoma and multiple myeloma and also for the treatment of solid tumors. It inhibits Cdk1, Cdk2 and Cdk4 and it blocks cells in both G1 and G2 phases. It is also a weaker inhibitor of PKC and EGFR (Senderowicz, A. M. et al.
J. Natl. Cancer Inst
2000, 92, 376-387).
WO9716447 (Mitotix) discloses the following compounds related to flavopiridol
Some of these compounds are stated to inhibit Cdk4.
WO9943675 and WO9943676 (Hoechst) disclose the following purine derivatives
which are stated to inhibit Cdk2 and Cdk4.
WO9833798 (Warner-Lambert) discloses the following pyridopyrimidines
These compounds are stated to inhibit the cyclin dependent kinases Cdk1, Cdk2, and Cdk4. Some of these compounds also inhibit the receptor tyrosine kinases PDGFR and EGFR, and the cellular Src protein kinase, c-Src.
WO9909030 (Warner-Lambert) discloses naphthyridinones
that inhibit PDGFR, FGFR, c-Src, and the cyclin dependent kinases Cdk1, Cdk2, and Cdk4.
WO0039101 (AstraZeneca) discloses diaminopyrimidines
that inhibit Cdk4 and FAK3.
WO0012485 (Zeneca) discloses diaminopyrimidines
that inhibit Cdk4 and FAK3.
WO9924416 (Bristol-Myers Squibb) discloses aminothiazole inhibitors of formula
The compounds inhibit Cdk1, Cdk2 and Cdk4.
WO9921845 (Agouron) discloses diaminothiazole inhibitors of Cdk1, Cdk2 and Cdk4, having the following structure
where R1 and R2 are ring systems. This patent application indicates that in cases where the R
2
ring system does not bear an ortho substituent, the compounds lack potency and selectivity as inhibitors of Cdk4.
Finally, WO0075120 (Agouron) discloses diaminothiazole inhibitors of protein kinases including VEGF-R, FGF-R, CDK complexes, TEK, CHK1, LCK, and FAK, having the following structure
It is desirable to provide small molecule inhibitors of Cdk4 that are selective against other Cdks. That is, the small molecule is significantly more inhibitory (at least 10 times, preferably 100 times) of Cdk4 than Cdk1 and Cdk2. This parameter is desirable because of the potential concomitant toxicity and other undesirable complications that may follow from inhibiting multiple targets. Thus, for purposes of this invention, the inhibition of Cdk2 and Cdk1 are monitored to determine the selectivity of the inhibition of Cdk4. A compound that exhibits selectivity against Cdk2 and Cdk1 is expected to have a better safety profile than a compound that is not selective between Cdk4, Cdk2 and Cdk1.
There continues to be a need for easily synthesized, small molecule compounds that are specific inhibitors of Cdk4 for the treatment or control of one or more types of solid tumors. It is an object of this invention to provide such compounds, compositions containing such compounds, and methods of using such compounds in the treatment or control of breast, colon, lung and prostate tumors.
SUMMARY OF THE INVENTION
The present invention is directed to novel diaminothiazoles capable of selectively inhibiting the activity of Cdk4. These compounds are useful in the treatment or control of cancer, in particular the treatment or control of solid tumors. In particular this invention is directed to a compound of formula
or the pharmaceutically acceptable salts or esters thereof, wherein
R
1
is selected from the group consisting of
H,
lower alkyl that optionally may be substituted with a group selected from OR
6
, cycloalkyl, and NR
7
R
8
,
cycloalkyl,
COR
9
, and
SO
2
R
10
;
R
2
is selected from the group consisting of
H,
F,
Cl
Chen Li
Ding Qingjie
Gillespie Paul
Kim Kyungjin
Lovey Allen John
Hoffmann-La Roche Inc.
Johnston George W.
Raymond Richard L.
Rocha-Tramaloni Patricia S.
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