Diamine oxidase and assay for rupture of amniotic membrane in pr

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436501, 436514, 436518, 436536, 436548, G01N 3353, G01N 33536, G01N 33543, G01N 33558

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active

052847497

ABSTRACT:
Assay for detection of a form of diamine oxidase which is present only in amniotic fluid and, more especially, for detection of amniotic membrane rupture by detection of the amniotic fluid diamine oxidase. The assay includes the steps of detecting the amniotic fluid diamine oxidase and distinguishing that from another form of diamine oxidase found in serum. The assay may be carried out on a sample of vaginal fluid from a pregnant female wherein the sample is subjected to the assay to detect the leakage or presence of amniotic fluid diamine oxidase. Purified forms of amniotic fluid diamine oxidase and serum diamine oxidase which is different from amniotic fluid diamine oxidase and found in serum are also described, together with a method of purifying both forms of diamine oxidase.

REFERENCES:
Watkins et al. Biosis Abstract No. 64031438 Ann. Clin Lab Sci. 7(3) 1977. 231-240.
Gahl et al. Biosis Ab. No. 75040200 Obstet Gynecol. 60(3) 279-304 (1982).
Oratore et al. FEBS Letters, 104(1) 154-156 (1979).

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