Diagnostics based on tetrazolium compounds

Details Diagnostics based on tetrazolium compounds Diagnostics based on tetrazolium compounds

1998-09-28

1999-05-11

Fonda, Kathleen K.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 14, 4352877, 4352878, 435962, 435970, 436110, C12Q1/32;1/54

Patent

active

059027312

ABSTRACT:
A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD or an NAD derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

REFERENCES:
patent: 4803158 (1989-02-01), Shigeta et al.
patent: 5510245 (1996-04-01), Magers
Lindquist et al. Rapid Determination of Toxic Cyanide in Blood. Clinical Chemistry 35 (4), pp. 617-619. (4/1989).
Tietz Textbook of Clinical Chemistry, 2nd Ed., ed. by C. Burtis et al., W.B. Saunders Co., Philadelphia, PA, 1994, pp. 974, 976-978 and 1174-1175.
KetoSite.RTM. test (available from GDS Diagnostics, Elkhart, IN.) 12/29/93.
Hemoglobin:Molecular, Genetic and Clinical Aspects, by Franklin H.Gunn and Bernard G. Forget, W.B. Saunders Co., Philadelphia, PA, 1968, pp. 638-644.
Glucometer Encore.TM. Blood Glucose Test Strips, Distributed by Miles Canada, Inc., Diagnostics Division, Etobicoke, Ontario M9W 1G6, 1992.

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