Diagnostic test for Staphylococcal mastitis in cattle

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 4, 435 29, 435 36, 435820, 435883, 436501, 436518, 436811, 530350, 530825, G01N 3353, G01N 33543, C12Q 114

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048493414

ABSTRACT:
The present invention relates to (1) an enzyme-linked immunosorbent assay (ELISA) for detection in milk of antibodies of any isotype which are specific for Staphylococcus aureus proteins in molecular weights ranging from 18,000 to 26,000 daltons, (2) a process for production and purification of said proteins, (3) a method of performing said ELISA utilizing said proteins and (4) use of said ELISA for detection of intramammary infection by S. aureus.

REFERENCES:
patent: 4425330 (1984-01-01), Norcross et al.
Davis A. et al., Journ Biol Chem., 252, No. 18:6544-6553, (1977).
Gudding, R., Acta Vet Scand., 21:242-255, (1980).

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