Diagnostic test for staphylococcal mastitis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 4, 435 29, 435 36, 435820, 435883, 436501, 436518, 436811, 530350, 530825, G01N 33569, C07K 1504

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active

051322102

ABSTRACT:
The present invention relates to (1) an enzyme-linked immunosorbent assay (ELISA) for detection in milk of antibodies of any isotype which are specific for Staphylococcus aureus proteins in molecular weights ranging from 14,000 to 26,000 daltons, (2) a process for production and purification of the proteins, (3) a method of performing the ELISA utilizing the proteins (4) use of the ELISA for detection of intramammary infection by S. aureus, (5) preparation of monoclonal and polyclonal antibodies against the selected fractions, (6) use of such antibodies of purification of infection specfic antigens, and (7) use of the antibodies in an ELISA for antibodies produced in an individual in response to infection by S. aureus.

REFERENCES:
patent: 4425330 (1984-01-01), Norcross et al.
patent: 4849341 (1989-07-01), Adams et al.
Davis A. et al., Journ. Biol Chem. 252 No. 18:6544-6553 (1977).
Gudding, R., Acta. Vet. Scand. 21:242-255 (1980).
Adams et al, "Staphylococcus aureus Antigens Reactive with Milk Immunoglobulins G of Naturally Infected Dairy Cows" J. Clin. Microbiol 26(6) pp. 1175-1180 Jun. 1988.
Sevier et al. "Monoclonal Antibodies in Clinical Immunology" Clin Chem 27(11) pp. 1797-1805 1981.
Oellerich, "Enzyme-Immunoassay: A Review" J. Clin chem Clin Biochem. 22 pp. 895-904 1984.

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