Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-11-22
1998-04-28
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 2, 435810, 435 792, 435974, 436164, 436172, 436536, 436811, 5303881, 5303413, G01N 3353, G01N 33555, G01N 33567, C07K 1600
Patent
active
057443173
ABSTRACT:
A method for distinguishing replicatively senescent T cells from replicatively non-senescent T cells in a cell population comprises the steps of: (1) providing a cell population of peripheral blood mononuclear cells; (2) reacting the cell population with a first monoclonal antibody specific for CD3 antigens which is present on all T cells, the antibody being labeled with a first detectable label, so that the monoclonal antibody binds to all T cells in the cell population; (3) simultaneously reacting the cell population with a second monoclonal antibody specific for CD28 antigen, the antibody being labeled with a second detectable label distinguishable from the first detectable label, so that the second monoclonal antibody binds to T cells positive for CD28; and (4) in the cell population of peripheral blood mononuclear cells, detecting the T cells that simultaneously react with the first monoclonal antibody and the second monoclonal antibody by observing the first detectable label bound to the cells simultaneously with the second detectable label bound to the cells, thereby distinguishing replicatively senescent T cells, which are CD28-negative, from replicatively non-senescent T cells, which are CD28-positive, and determining any of: (a) the number of replicatively senescent T cells in the cell population; (b) the number of replicatively non-senescent T cells in the cell population; or (c) the proportion of replicatively senescent T cells to replicatively non-senescent T cells in the cell population. The method can also be used to separate and isolate cells showing immunological senescence from non-senescent cells.
REFERENCES:
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Chin Christopher L.
Nguyen Bao-Thuy L.
The Reagents of The University of California
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