Textiles: fiber preparation
Patent
1976-06-04
1977-08-02
Naff, David M.
Textiles: fiber preparation
195 99, 260403, 2604045, 2606065P, 260621N, 424 7, G01N 3114
Patent
active
040393887
ABSTRACT:
An artificial substrate consisting of a 2-alkanoylamino-4-nitrophenyl phosphorylcholine hydroxide is used for determining the sphingomyelinase activity in an extract of human cells or tissues by incubating the substrate together with the extract, whereby sphingomyelinase-catalyzed hydrolysis of the substrate into the corresponding 2-alkanoylamino-4-nitrophenol and phosphorylcholine takes place in an amount proportional to the sphingomyelinase activity in the extract. Alkalinization of the 2-alkanoylamino-4-nitrophenol thereby produced to convert it into the alkali salt results in the development of a bright yellow color whose intensity is proportional to the amount of the substrate that has been hydrolyzed. Measurement of the intensity of the yellow color, for example, by means of a simple colorimeter or photometer, provides an accurate indication of the sphingomyelinase activity in the extract.
REFERENCES:
gal et al., A Practical Chromogenic Procedure for the Detection of Homozygotes and Heterozygons Carriers of Niemann-Pick Disease, The New England Journal of Medicine, vol. 293, No. 13, 1975, (pp. 632-636).
Brady, R. O., Hereditary Diseases-Causes, Cures, and Problems, Angem. Chem. Internat. Edit. vol. 12, No. 1, 1973, (pp. 1-11).
Brady Roscoe O.
Gal Andrew E.
Naff David M.
The United States of America as represented by the Government
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