Chemistry: analytical and immunological testing – For preexisting immune complex or auto-immune disease
Patent
1995-05-23
1998-07-28
Knode, Marian C.
Chemistry: analytical and immunological testing
For preexisting immune complex or auto-immune disease
435 721, 436518, 436811, 436827, G01N 33564, G01N 33543
Patent
active
057862214
ABSTRACT:
An in vitro diagnostic test for analyzing body fluid so as to obtain an indication of the risk of developing insulin dependent diabetes-mellitus (IDDM) is provided. A solid phase containing immobilized pancreatic antigens is contacted with a test sample. The solid phase includes a water insoluble polymer carrier, a lectin attached to the water insoluble polymer, and mammal derived pancreatic proteins. The pancreatic proteins include pancreatic antigens which are reactive to islet cell autoantibodies which are routinely detected in the blood of patients who have recently been diagnosed as having insulin dependent diabetes mellitus (IDDM). A test sample is incubated with the solid phase containing immobilized pancreatic antigens for a sufficient time to allow a reaction between the immobilized pancreatic antigens and autoantibodies to the pancreatic antigens in the sample to bind the autoantibodies to the solid phase. Detectably labelled molecules which are selectively reactive to human IgG are then added and incubated with the solid phase for a sufficient time to allow the reaction of the labelled molecules with the bound antibodies to the pancreatic antigens to thereby attach the labelled molecules solid phase.
REFERENCES:
Tsuji et al., Binding of islet cell cytoplasmic antibody from insulin-dependent diabetes mellitus patients and plant lectin (Maclura pomifera agglutinin) to pancrease islet cells, Biol. Abstr. 83(6):54630, 1987.
Kitagawa et al., Detection of islet-cell-surface antibodies by using 125l-labeled wheat germ agglutinin (125l-WGA) bound islet-cell protein, Chem. Abs. 103:176674g, 1985.
Baekkeskov et al., Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins, Nature 298:167-169, 19982.
Doyle et al., Applications of Lectins in Microbiology, ASM News 55(12):655-658, 1989.
Allergy Immunotechnologies, Inc., Isletest-ICA Qualitative Test for Detection of Circulating Antibodies Against Islet Cell Atnigens, 1989.
Ma Wai-Sai
Rao Srinivasa
Biomerica, Inc.
Knode Marian C.
Quinton James A.
Wortman Donna C.
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