Diagnostic test for hepatitis B virus

Details Diagnostic test for hepatitis B virus Diagnostic test for hepatitis B virus

1981-03-31

1985-12-31

Padgett, Ben R.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436504, 436804, 436808, 436810, 436811, 436815, 436820, 435 6, 4351723, 435235, 435317, 435948, 935 78, 206569, C12N 1500, G01N 3350

Patent

active

045621594

ABSTRACT:
A method and test kit are disclosed for detecting the presence of hepatitis B virus in a test specimen containing at least a portion of the DNA of the virus. A test reagent comprises cloned hepatitis B virus-DNA that has been repurified by treatment with a restriction enzyme and labelled to high specific activity with a radioactive label. The sample to be tested is fixed to a solid matrix, incubated in the presence of the test reagent under hybridization conditions and detected by hybridization to the labelled DNA probe. The uncombined HBV-DNA (labelled) is removed from the substrate, and the hybridized HBV-DNA determined by scintillation counting or by autoradiography of the substrate.

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