Diagnostic test for collie eye anomaly

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Details

C435S091200, C536S023500, C536S024310, C536S024330

Reexamination Certificate

active

07462455

ABSTRACT:
The invention relates to a method for identifying dogs which are genetically normal, heterozygous for, or homozygous for the mutation primarily responsible for Collie eye anomaly (CEA). The method comprises the steps of obtaining a biological sample from a dog and testing DNA in the biological sample for the presence or absence of a 7.8 kilobase deletion within chromosome37in which the CEA mutation is located. No deletion is indicative of a normal dog. A deletion on one allele of chromosome37is indicative of a dog that is heterozygous for the CEA mutation. A deletion in both alleles of chromosome37are indicative of a dog that is homozygous for the CEA mutation. Also provided is a kit for identifying a dog as normal, heterozygous for, or homozygous for the CEA mutation.

REFERENCES:
Lowe et al., Linkage Mapping of the Primary Disease Locus for Collie Eye Anomaly, Genomics 82 (2003) 86-95.

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