Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-09-14
1998-10-27
Knode, Marian C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
436518, 436811, C12Q 170, G01N 33543
Patent
active
058276467
ABSTRACT:
The present invention is directed to diagnostic reagents for the detection of antibodies against the Epstein Barr Virus and a method for the detection of antibodies to the Epstein Barr Virus in a sample.
The diagnostic reagents according to the invention comprise a combination of at least part of an Epstein Barr viral structural protein, preferably a Epstein Barr VCA-protein or an Epstein Barr MA protein, and at least part of an Epstein Barr EBNA protein. Preferably, the VCA-protein is VCA-p18 protein, the MA-protein is MA-gp350/220 protein and the EBNA-protein is EBNA-1 protein.
It has been found that the combination of a VCA-protein or a MA-protein, and an EBNA protein, into a single diagnostic assay yields an EBV-antibody detection method with greater sensitivity and accuracy than current methods.
REFERENCES:
patent: 5122448 (1992-06-01), Vaughan et al.
P.J. Durda et al., "An Enzyme-Linked Immunosorbent Assay for the Measurement of Human IgA Antibody Responses to Epstein-Barr Virus Membrane Antigen", Intervirology 1993, 36:11:11-19.
W.M.J. van Grunsven et al., "Localization and Diagnostic Application of Immunodminant Domains of the BFRF3-Encoded Epstein-Barr Virus Capsid Protein" The Journal of Infectious Diseases, 1994, 170:13-19.
Dobec, M., "A New Combi Test for Simultaneous Detection of Antibodies to Viral Capsid, Early and EBNA Antigens of Epstein-Barr Virus," Zentralblatt Fur Bacteriologie, vol. 278, No. 4, Jun. 1993, pp. 553-561.
Geltosky, J.E. et al., "Use of a Synthetic Peptide-Based ELISA for the Diagnosis of Infectious Mononucleosis and Other Diseases," Journal of Clinical Laboratory Analysis, vol. 1, 1987, pp. 153-162.
van Grunsven, W.M.J. et al., "Gene Mapping and Expression of Two Immunodominant Epstein Barr Virus Capsid Proteins," Journal of Virology, vol. 67, No. 7, Jul. 1993, pp. 3908-3916.
Qualtiere et al., Epitope mapping of the major Epstein-Barr virus outer envelope glycoprotein gp350/220, J. Gen. Virol. 68(Pt. 2):535-543, 1987.
Middeldorp Jaap Michiel
van Grunsven Wouterus Marinus Johannes
Akzo Nobel N.V.
Blackstone William M.
Gormley Mary E.
Knode Marian C.
Wortman Donna C.
LandOfFree
Diagnostic reagents for the detection of antibodies to EBV does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Diagnostic reagents for the detection of antibodies to EBV, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic reagents for the detection of antibodies to EBV will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1612739