Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-05-02
1988-08-30
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 7, 435810, 436501, 436808, 536 26, 536 27, 536 28, 935 77, 935 78, C12Q 168, G01N 3353, G01N 33566
Patent
active
047676990
ABSTRACT:
A reagent complex is disclosed containing (1) a probe polynucleotide having a target binding region complementary to a target nucleotide sequence and (2) a signal strand polynucleotide bound by base pairing to a portion of the target binding region. The signal strand is displaced from the reagent complex by the target nucleotide sequence and separated from intact reagent complexes. A digestable ribonucleotide segment of the displaced signal strand, such as a 3' , terminal segment, is digested to ribonucleotide phosphates, which are phosphorylated to ATP. The ribonucleotide phosphates or ATP are detected.
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Diamond Steven E.
Vary Calvin P. H.
Wolfman Neil M.
Allied Corporation
Doernberg Alan M.
Spiegel Jack
Warden Robert J.
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