Diagnostic reagent holder and method

Details Diagnostic reagent holder and method Diagnostic reagent holder and method

1975-11-03

1976-12-28

Reese, Robert M.

Chemistry: physical processes

Physical processes

Crystallization

23253R, 23253TP, 23259, 128 2W, 1951035R, 195127, 356244, 356246, G01N 100, G01N 2124, G01N 3316

Patent

active

039999483

ABSTRACT:
A diagnostic reagent holder specifically adapted for the detection of an unknown quantity of a biologically derived sample suitably by radioimmunoassay, fluorometric detection or by spectrophotometry. The holder comprises an elongate shaft with a support surface adjacent one end of the shaft. Diagnostic reagent is covalently attached to a self-supporting film such as a disc and firmly secured to the support. The diagnostic reagent, e.g., antibody, is stirred during incubation in contact with the sample to be detected, e.g., antigen, in solution. In a "sandwich" technique labelled substance (e.g., fluorochrome-labelled antibody) is reacted with the sample on the film followed by thorough washing. Then, the holder is slid into a novel viewing housing which precisely positions the film at a window for viewing as with a fluorometer. To avoid loss of labelled substance during handling, and especially during positioning in the viewing housing, one or more protective protrusions are provided around the periphery of the support surface.

REFERENCES:
patent: 2749797 (1956-06-01), Harks
patent: 3443903 (1969-05-01), Haack et al.
patent: 3507269 (1970-04-01), Berry
patent: 3526480 (1970-09-01), Findl et al.
patent: 3881993 (1975-05-01), Freake et al.
patent: 3901657 (1975-08-01), Lightfoot
patent: 3904373 (1975-09-01), Harper
patent: 3907503 (1975-09-01), Betts et al.

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