Diagnostic probes for pneumocystis carini

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2374, 536 2432, 536 2433, 935 8, 935 78, C12Q 168, C12P 1934, C07H 2104

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057766800

ABSTRACT:
The present invention pertains to a method for diagnosing for Pneumocystis carinii by detecting the presence of a nucleic acid sequence containing the 26S rRNA gene specific for Pneumocystis carinii. More particularly, this invention relates to a method for diagnosing for Pneumocystis carinii which comprises amplifying a sample of DNA from Pneumocystis carinii by polymerase chain reaction (PCR) using species specific primers and detecting the PCR products with species specific radioactive or non-radioactive oligonucleotide probes. This invention also relates to a method for diagnosing for various species of Pneumocystis carinii by detecting the presence of a nucleic acid sequence containing the particular 16S or 26S rRNA gene sequence specific for that species of Pneumocystis carinii.

REFERENCES:
Erlich et al, Science (1991) 252:1643-1651.
Regensburger, J. Gen Microb (1988) 134:1197-1204.
Williams BioTechniques (1989) 17:762-768.
White et al PCR Protocols: A Guide to Methods & Applications, 1990, Innis et al, Eds, pp. 315-322.

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