Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-07-21
1998-07-07
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 2374, 536 2432, 536 2433, 935 8, 935 78, C12Q 168, C12P 1934, C07H 2104
Patent
active
057766800
ABSTRACT:
The present invention pertains to a method for diagnosing for Pneumocystis carinii by detecting the presence of a nucleic acid sequence containing the 26S rRNA gene specific for Pneumocystis carinii. More particularly, this invention relates to a method for diagnosing for Pneumocystis carinii which comprises amplifying a sample of DNA from Pneumocystis carinii by polymerase chain reaction (PCR) using species specific primers and detecting the PCR products with species specific radioactive or non-radioactive oligonucleotide probes. This invention also relates to a method for diagnosing for various species of Pneumocystis carinii by detecting the presence of a nucleic acid sequence containing the particular 16S or 26S rRNA gene sequence specific for that species of Pneumocystis carinii.
REFERENCES:
Erlich et al, Science (1991) 252:1643-1651.
Regensburger, J. Gen Microb (1988) 134:1197-1204.
Williams BioTechniques (1989) 17:762-768.
White et al PCR Protocols: A Guide to Methods & Applications, 1990, Innis et al, Eds, pp. 315-322.
Leibowitz Michael J.
Liu Yong
Muccino Richard R.
Myers Carla J.
University of Medicine & Dentistry of New Jersey
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