Diagnostic primers and probes

Details Diagnostic primers and probes Diagnostic primers and probes

1995-06-08

1997-08-05

Campbell, Eggerton A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2433, C07H 2102, C07H 2104

Patent

active

056544162

ABSTRACT:
An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.

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