Diagnostic method for the identification of foetal DNA in a...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091100, C435S091200

Reexamination Certificate

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07083924

ABSTRACT:
A method for the identification of foetal DNA in a maternal DNA-containing sample such as a blood or vaginal sample, said method comprising (a) isolating DNA from said sample, (b) subjecting said DNA to exonucleolytic digestion by an enzyme so as to remove end regions of each DNA molecule, and (c) detecting the presence of a DNA sequence remaining in foetal DNA but absent form maternal DNA as a result of said digestion process. Once identified, the foetal DNA can be subject to diagnosis for example to detect chromosomal/DNA abnormalities, including in particular aneuploides such as foetal trisomy 21.

REFERENCES:
patent: 2003/0186287 (2003-10-01), Lin et al.
patent: 2004/0018509 (2004-01-01), Bianchi
patent: 0430402 (1991-06-01), None
patent: 94/02646 (1994-02-01), None
patent: 98/39474 (1998-09-01), None
Verma et al., “Rapid and simple prenatal DNA diagnosis of Down's syndrome”, The Lancet, vol. 352, (1998), pp. 9-12.
Poon et al., “Prenatal detection of fetal Down's syndrome from maternal plasma”, The Lancet, vol. 356, (2000), pp. 1819-1821.
Youngren, Kjell et al.; “Synchrony In Telomers Length of the Human Fetus”;Human Genetics; vol. 102, No. 6; Jun. 1998; pp. 640-643; (XP002200862).
Iwama, Hiroshi et al.; “Telomeric Length and Telomerase Activity Vary With Age in Peripheral Blood Cells Obtained from Normal Individuals”;Human Genetics; vol. 102, No. 4; Apr. 1998; pp. 397-402; (XP002200863).

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