Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing
Reexamination Certificate
2003-09-17
2010-06-08
Swartz, Rodney P. (Department: 1645)
Drug, bio-affecting and body treating compositions
In vivo diagnosis or in vivo testing
C424S009200, C424S130100, C424S158100, C424S184100, C424S234100, C424S248100, C424S085200, C424S085400, C424S085500, C435S386000
Reexamination Certificate
active
07731937
ABSTRACT:
The present invention is intended to provide a diagnostic method forparatuberculosisby which an animal infected withMycobacterium aviumsubsp.Paratuberculosiscan be diagnosed at a high sensitivity in the sub-clinical infection stage before the specific antibody level begins to increase and a large number of specimens can be treated. The present invention provides: a diagnostic method forparatuberculosischaracterized by collecting the blood of a subject animal, adding an anti-IL-10 antibody and aMycobacterium aviumsubsp.Paratuberculosisantigen to the collected blood followed by culture, and then measuring the amount of produced IFNγ in the blood; a diagnostic method forparatuberculosischaracterized in that the amount of produced IFNγ in blood is measured by the IFNγ ELISA method; and a diagnostic method for mycobacterial infection characterized by collecting the blood of a subject animal, adding an anti-IL-10 antibody and a mycobacterial antigen to the collected blood followed by culture, and then measuring the amount of produced IFNγ in the blood.
REFERENCES:
patent: 5679347 (1997-10-01), Porcelli et al.
Koets, A.P., et al. Heat-shock protein-specific T-cell response in various stages of bovine paratuberculosis. Veterinary Immunology and Immunopathology. vol. 70, No. 1-2, pp. 105-115, 1999.
Cole, S.T. Comparative mycobacterial genomics as a tool for drug target and antigen discovery. Eur. Respir. J., vol. 20, Suppl. 36, pp. 78s-86s, 2002.
Lind, A. Mycobacterial species: immunological classification, in, The Mycobacteria, ed. G.P. Kubica and L.G. Wayne, Marcel Dekker, Inc., New York, pp. 67-82, 1984.
Merkel, R.S. Paratuberculosis, in, The Mycobacteria, ed. G.P. Kubica and L.G. Wayne, Marcel Dekker, Inc., New York, pp. 1237-1249, 1984.
Coussens et al.“Gene expression profiling of peripheral blood monouclear cells from cattle infected withMycobacterium paratuberculosis”, Infection and Immunity, vol. 70, No. 10 Oct. 2002.
Weiss et al., “Differential responses of bovine macrophages toMycobacterium aviumsubsp.Paratuberculosis”, Infection and Immunity, vol. 70, No. 10 (2002) pp. 5556 to 5561.
Olsen et al. “Elevated antibody responses in patients with Crohn's disease against a 14-kDa secreted protein purified fromMycobacterium aviumsubsp.Paratuberculosis”, Scandinavian Journal of Immunology, vol, 53. No. 2 (2001). pp. 198 to 203.
Buza Joram Josephat
Hikono Hirokazu
Momotani Eiichi
Mori Yasuyuki
Antonelli, Terry Stout & Kraus, LLP.
Incorporated Administrative Agency National Agriculture and Bio-
Swartz Rodney P.
LandOfFree
Diagnostic method for paratuberculosis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Diagnostic method for paratuberculosis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic method for paratuberculosis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4241308