Chemistry: analytical and immunological testing – Oxygen containing – Carbonyl – ether – aldehyde or ketone containing
Patent
1992-09-04
1995-10-10
Redding, David A.
Chemistry: analytical and immunological testing
Oxygen containing
Carbonyl, ether, aldehyde or ketone containing
436161, 436173, 436175, 514 52, G01N 3300, A01N 4304
Patent
active
054570557
ABSTRACT:
A method of diagnosing cobalamin deficiency in warm-blooded animals is described. In one embodiment, this method comprises the measurement of methylmalonic acid levels in cerebral spinal fluid to determine if such levels exceed normal range. A finding of elevated levels indicates a cobalamin deficiency. In another embodiment, the method comprises the measurement of methylmalonic acid levels in cerebral spinal fluid and serum. A finding that the ratio of methylmalonic acid in cerebral spinal fluid to that in serum is above normal is indicative of a cobalamin deficiency. Additionally, a method for assessing the effectiveness of cobalamin therapy is described. The effectiveness of therapy is assessed by determining whether cerebral spinal fluid levels of methylmalonic acid have returned to normal, or by determining whether the ratio of methylmalonic acid in cerebral spinal fluid to that in serum has returned to normal range. A method for distinguishing cobalamin deficiency from renal failure in warm-blooded animals having excessive serum MMA levels is also described. This method comprises determining the ratio of CSF MMA to serum MMA. A finding that the CSF MMA/serum MMA ratio is above normal or elevated is indicative of the presence of Cbl deficiency, whereas a finding that the CSF MMA/serum MMA ratio is below normal is indicative of renal failure.
REFERENCES:
patent: 4940658 (1990-07-01), Allen et al.
Lindenbaum, et al. (1988) "Neuropsychiatric Disorders Caused by Cobalamin Deficiency in the Absence of Anemia or Macrocytosis" New Eng. J. Med. 318:1720-1728.
Norman et al. (1982) "Cobalamin (Vitamin B.sub.12) Deficiency Detection by Urinary Methylmalonic Acid Quantitation" Blood 59(6):1128-1131.
Lindenbaum et al. (1988) "New Assays for Cobalamin Deficiency: Getting Better Specificity" Laboratory Management 26:41-44.
Marcell et al. (1985) "Quantitation of Methylmalonic Acid and Other Dicarboxylic Acids in Normal Serum and Urine Using Capillary Gas Chromatography-Mass Spectrometry" Anal. Biochem. 150:58-66.
Stabler et al. (1986) "Assay of Methylmalonic Acid in the Serum of Patients with Cobalamin Deficiency Using Capillary Gas Chromatography-Mass Spectrometry" J. Clin. Invest. 77:1606-1612.
Leupold (1977) "Methylmalonacidurie" Klin. Wschr. 55:57-63.
Oberholzer et al. (1965) "Methylmalonic Aciduria" Arch. Dis. Childh. 42:492-504.
Morrow and Barness (1969) "Studies in a Patient With Methylmalonic Acidemia" J. Pediatr. 74(5):691-698.
Stabler et al. (1989) "Marked Elevation of Methylmalonic Acid (MMA) in Cerebral Spinal Fluid (CSF) of Patients With Cobalamin (Cbl) Deficiency" Clin. Res. 37(2):550A.
Sprinkle et al. (1969) "A Simple Method for the Determination of Methylmalonic Acid by Gas Chromatography" Clin. Chim. Acta 24:476-478.
Barness, L. A. (1967) "Vitamin B.sub.12 Deficiency With Emphasis on Methylmalonic Acid as a Diagnostic Aid" Amer. J. Clin. Nutrition 20(6):573-577.
Allen Robert H.
Lindenbaum John
Stabler Sally P.
Redding David A.
The University of Colorado Foundation
LandOfFree
Diagnostic method for cobalamin deficiency does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Diagnostic method for cobalamin deficiency, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic method for cobalamin deficiency will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2310077