Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-11-30
1996-02-20
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 793, 435 794, 435 795, 436518, 436804, 436811, G01N 3353, G01N 33537, G01N 33543
Patent
active
054928125
ABSTRACT:
The present invention is directed to methods and kits for diagnosing, Alzheimer's disease. The invention is based, in part, on the discovery that proteolytic fragments of the amino and carboxy terminal amino acid residues of tau-proteins are released from the neurofibrillary tangles associated with the disease and can be detected in body fluids outside the brain. The tau-proteins will be purified or chemically synthesized and peptide fragments of the amino terminal and carboxy terminal regions will be obtained proteolytically or synthesized chemically and will be used in generating tau specific antibodies for use in diagnostic kits for the detection of Alzheimer's disease. These diagnostic kits will be used in screening the body fluids of individuals for the presence of tau-peptide fragments. Alternatively, the tau-peptides themselves may be used in diagnostic kits for screening the body fluids of individuals for the presence of circulating autoantibodies.
REFERENCES:
Kosik et al, "Epitopes That span the Tau Molecule Are Shared with Paired Helical Filaments", Neurons 1:817-825 (Nov. 1988).
Singh et al, "Detection of Brain Autoantibodies in The Serum of Patients With Alzheimer's Disease But Not Down's Syndrome", Immunol. Lett., 12:277-280 (1986).
Gaskin et al. "Patients with Clinically Diagnosed Senile Dementia of the Alzheimer Type (SDAT) Make Autoantibodies That React with Neurofibrillary Tangles", Clin. Res., 34(2):669 A (1986).
Grundke-Iqbal et al, "Microtubule-associated Protein Tau", J. Biol. Chem., 261(13):6084-6089 (May 5, 1986).
Foley et al. "Evidence for the presence of antibodies to cholinergic neurons in the serum of patients with Alzheimer's disease", J. Neurol., 235:466-471 (1988).
Morris et al, "The Consortium to Establish a Registry for Alzheimer's Disease (CERAD)", Neurology 39:1159-1165 (1989).
Wolozin et al. "Alzheimer-Related Neuronal Protein A68: Specificity and Distribution", Ann. Neurol., 22:521-526 (1987).
Ksiezak-Reding et al, "Mapping of the Alz-50 Epitope in Microtubule-Associated Proteins Tau", J. Neurosci. Res., 25:412-419 (1990).
Ksiezak-Reding et al., 1988, "Immunochemical and biochemical characterization of .tau. proteins in normal and Alzheimer's disease brains with Alz 50 and Tau-1", J. Biol. Chem. 263(17):7948-7953.
Kingsley et al., 1988, "Human antibodies to neurofibrillary tangles and astrocytes in Alzheimer's disease", Neuroimmunol. 19:89-99.
Gaskin et al., 1987, "Autoantibodies to neurofibrillary tangels and brain tissue in Alzheimer's disease: Establishment of Epstein-Barr Virus-transformed antibody-producing cell lines", J. Exp. Med. 165:245-250.
Iqbal et al., 1989, "Laboratory Diagnostic tests for Alzheimer's disease", Prog. Clin. Biol. Res. 317:679-687.
Duffy Patricia A.
Provost, Fellows and Scholars of Trinity College
Scheiner Toni R.
LandOfFree
Diagnostic method for Alzheimer's disease by screening for tau-p does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Diagnostic method for Alzheimer's disease by screening for tau-p, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic method for Alzheimer's disease by screening for tau-p will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1355535