Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1999-02-22
2000-09-26
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 4353201, 435195, 435810, 536 231, 536 2431, 536 2433, C12Q 168, G01N 1934, C12N 914, C12N 1500, C07H 2102, C07H 2104
Patent
active
061241008
ABSTRACT:
The invention provides a diagnostic method and kits for SCA III syndrome. The method comprises attaching a portion of SCA III gene containing copies of 73 trinucleotide(CAG) repeat units to a substrate; amplifying a DNA segment containing copies of the trinucleotide repeat units from the genomic DNA of a testee using two labeled primers under the suitable condition for carrying out polymerase chain reaction (PCR); hybridizing the gene with the PCR product by amplifying DNA segment; and analyzing the results of the hybridization. A SCA III patient can be effectively diagnosed by examining the increase extent of the number of the TNR characteristic of the disease-associated gene with the aid of reverse dot hybridization technique or PCR-MPH. Useful for the diagnosis is a kit which comprises the substrate, two primers with a detectable label, which are used to amplify a DNA segment containing copies of the trinucleotide repeat unit from the genomic DNA of a testee, a buffer and a polymerase for polymerase chain reaction, a buffer for gene hybridization and an enzyme for color detection.
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Samsung Fine Chemicals Co. Ltd.
Wilder C.
Zitomer Stephanie W.
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