Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-06-10
2000-04-04
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 724, 435 4, 435 2, 436 18, 436 17, C12Q 128, C12Q 100, G01N 3353
Patent
active
060460199
ABSTRACT:
Methods for detecting granulocytes and for providing an at least semiquantitative indication of granulocyte level and kits for implementing those methods. A blood sample is drawn, and components that might interfere with the assay are removed and/or neutralized. Granulocytes present in the sample are then disrupted to release intracellular myeloperoxidase, and the sample is contacted with a peroxide and a chromogenic donor dye. The myeloperoxidase enzyme catalyzes hydrogen peroxide-involved reactions which result in the dye changing color if, and to the extent that, granulocytes are present in the blood sample.
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Griswold et al., Method for Quantification of Myocardial Infarction and Inflammatory Cell Infiltration in Rat Cardiac Tissue, Journal of Pharmacological Methods 20, 1988, pp. 225-235.
Mullane et al., Myeloperoxidase Activity as a Quantitative Assessment of Neutrophil Infiltration into Ischemic Myocardium, Journal of Pharmacological Methods 14, 1985, pp. 157-168.
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Procedure No. 380, Sigma Diagnostics, Clinical Technical Service, St. Louis, Missouri.
Goumeniouk Alexander P.
Richards B. G.
Leary Louise N.
Multer Richard D.
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