Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-06-09
1995-01-24
Fleisher, Mindy B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 2433, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500
Patent
active
053842421
ABSTRACT:
Amplification, replication or detection of a predetermined target nucleic acid can be carried out using a unique primer composition. This composition comprises and aqueous mixture of a first oligonucleotide primer which is substantially complementary to a first nucleic acid sequence of the target, but which is suspected of having one or more mismatches with the target at or near its 3' end. Also included in the composition is one or more additional primers which are complementary to a nucleic acid sequence of the target. This sequence is either: (i) inclusive of only a portion of the first nucleic acid sequence, (ii) immediately adjacent to the first nucleic acid sequence, or (iii) removed from the first sequence by one or more bases, but which additional primer is capable of forming a primer extension product complementary to the first sequence. These composition components can be supplied as part of a diagnostic test kit which can include other regents if desired.
REFERENCES:
patent: 4089747 (1978-05-01), Bruschi
patent: 4683195 (1987-01-01), Mullis et al.
patent: 4683202 (1989-07-01), Mullis
patent: 4988617 (1991-01-01), Landegren et al.
Clavel et al, Nature, vol. 324, Dec. 1986, pp. 691-695.
Saiki et al, Science, vol. 239, Jan. 29, 1988, pp. 487-491.
Matthews et al, Anal. Biochem, vol. 169, Feb. 1988, pp. 1-25.
Mullis et al, Cold Spring Harbor Symposia, 1986, Cold Spring Harbor Lab, pp. 263-273.
Eastman Kodak Company
Fleisher Mindy B.
Tucker James L.
LandOfFree
Diagnostic kit, primer composition and their use for replication does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Diagnostic kit, primer composition and their use for replication, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic kit, primer composition and their use for replication will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1468209