Chemistry: analytical and immunological testing – For preexisting immune complex or auto-immune disease
Reexamination Certificate
2007-08-28
2007-08-28
Priebe, Scott D. (Department: 1633)
Chemistry: analytical and immunological testing
For preexisting immune complex or auto-immune disease
C530S350000, C530S387100, C530S387200, C530S387900, C530S388100, C530S358000, C435S007100
Reexamination Certificate
active
10877683
ABSTRACT:
As anti-RNA polymerase (RNAP) antibodies are detected with high frequency in patients suffering from cutaneous scleroderma where skin sclerosis progresses rapidly, supervenes scleroderma renal crisis at a high rate, and associates with clinical entities whose prognoses are extremely bad, it is intended to provide a convenient method of detecting an anti-RNAP antibodies, which is extremely useful in diagnosing and classifying clinical entities of scleroderma, and predicting organ failure, in particular scleroderma renal crisis. In order to identify an epitope recognized commonly by anti-RNAP antibodies, the full length of RPC62 and a partial fragment of RPC155, that are 2 subunits of 62-kDa and 155-kDa of RNAP III, are expressed inEscherichia colias recombinant proteins, and the reactivities to sera positive and negative to anti-RNAP antibody from patients suffering from scleroderma are examined by immunoblotting method to confirm that an epitope recognized by anti-RNAP antibodies in the sera from the patients suffering from scleroderma exists in 891 to 1020 amino acid residues of RPC155.
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Burkhart Michael
Frommer & Lawrence & Haug LLP
Keio University
Kowalski Thomas J.
Priebe Scott D.
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