Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1990-09-25
1992-08-11
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
435 6, 435 74, 435287, 435817, 435174, 435176, 435177, 436149, 436501, 436518, 436524, 436525, 436527, 436 73, 436806, 422 8202, 422 90, 422 98, 42218604, 42218615, 42218616, 42218622, 42218626, 204193, C12N 1114, C12N 1100, G01N 3353, G01N 33563
Patent
active
051378272
ABSTRACT:
A method is disclosed for detecting the occurrence of a binding or complex-forming reaction between specific substances by utilizing the binding reaction to modify an electrical circuit, and then measuring a change in the electrical state of the circuit. A diagnostic element useful in such a method includes a layer of a biogenic substance, such as an antigen, coated onto a non-conductive base between a pair of electrical conductors superposed on the base. Antibodies which react with the antigen are treated so that they become bound to fine electrically conductive, metallic particles. The electrically conductive particles having antibody bound thereto are then added to the antigen layer deposited on the base and allowed to react therewith. Electrically conductive particles are thereby bound to the base due to the binding reaction between the antigen and antibody to thereby form aggregates of electrically conductive particles which modify the circuit. The method of the invention is highly useful for the detection of antigens and antibodies in the blood serum of a human patient.
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Mroczkowski Susan J.
Siegesmund Kenneth A.
Yorde Donald E.
Kepplinger Esther L.
Midwest Research Technologies, Inc.
Scheiner Toni R.
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