Diagnostic compositions, elements, methods and test kits for amp

Details Diagnostic compositions, elements, methods and test kits for amp Diagnostic compositions, elements, methods and test kits for amp

1995-06-08

1998-09-22

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Apparatus

435 6, A01N 102, C12Q 168

Patent

active

058112952

ABSTRACT:
An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5008182 (1991-04-01), Sninsky et al.
patent: 5079351 (1992-01-01), Sninsky et al.
patent: 5147777 (1992-09-01), Sutton et al.
patent: 5173260 (1992-12-01), Zander et al.
patent: 5176995 (1993-01-01), Sninsky et al.
patent: 5196305 (1993-03-01), Findlay et al.
patent: 5229297 (1993-07-01), Schnipelsky et al.
Urdea et al, Nucleic Acid Res 16:4937-4956 (1988).
Nedjar e al., J. Virol. Meth., 35, pp. 297-304 (1991).
Jackson et al., N. England J. Med., 322 (4), pp. 217-222 (1990).
PCR Protocols: A Guide to Methods & Applications Chp. 40, Kellog & Kwok, Academic Press 1990.
Hewlett et al., Oncogene, 4, pp. 1149-1151 (1989).
Coutlee et al., Molecular & Cellular Probes, 3, pp. 241-259 (1991).
Chow, J. Clin. Microbiol., 30(9), pp. 2307-2310 (1992).
Frank et al., Modern Patholoty, 5(4), pp. 449-454 (1992).
Sokol et al., J. Clin. Microbiol., 30(4), pp. 839-844 (1992) (1991).
Brytting et al., J. Viro. Methods, 32, pp. 127-138 (1991).
Demmler et al., J. Infect. Diseases, 158(6), pp. 1177-1184 (1988).
Porter-Jordan et al., J. Med. Virol., 30, pp. 85-91 (1990).
Fenner et al., J. Clin. Microbiol., 29(11), pp. 2621-2622 (1991).
Hsia et al., J. Clin. Microbiol., 27(8), pp. 1802-1809 (1989).
Gibbs et al., Genomics, 7, pp. 235-244 (1990).
Mullis et al. Cold Spr. Hrb. Syn. L1; pp. 263-273 (1986).
Matthews et al., Anal. Biochem., 169, pp. 1-25 (1988).
Spaete et al., Virology, 167, pp. 207-225 (1988).
Vandenvelde et al., J. Virol. Metho., 30, pp. 215-228 (1990).

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