Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-07-15
1997-07-29
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 911, 435 912, 435 913, 536 245, C12Q 168, C12P 1934, C07H 2104
Patent
active
056521073
ABSTRACT:
There are provided nucleic acid hybridization assays for RNA targets using RNA binary probes and a ribozyme ligase that is a stringent RNA-directed RNA ligase. Preferred assays include exponential amplification for signal generation. Tetrahymena ribozyme ligase is a preferred ligase for use in this invention. It may be tethered to hold it close to the ligation junction. One assay according to this invention is a "tethered ligase chain reaction." Also provided are kits for performing assays according to the invention.
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Kramer Fred R.
Landegren Ulf D.
Lizardi Paul M.
Szostak Jack W.
Tyagi Sanjay
Elliott George C.
Fredman Jeffrey
The Public Health Research Institute of the City of New York, In
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