Diagnostic assay for inhibitor of tissue-type and urokinase-type

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 793, 435 13, 435 692, 436518, G01N 3353

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active

056291604

ABSTRACT:
A biological reagent and diagnostic system for the detection and quantitation of endothelial plasminogen activator inhibitor (PAI) are disclosed, as are substantially pure, recombinant human endothelial PAI, its biologically pure gene and a vector for cloning the gene and expressing a gene product.

REFERENCES:
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Sprengers, E. D., et al. "Evidence for the presence of two different fibrinolytic inhibitors in human endothelial cell conditioned medium." Biochim. Biophys. Acta 801 : 163-70, 1984.
Pheleps, M. et la. "Human endothelial cells produce a plasminogen activator inhibitor and a tissue type plasminogen activator-inhibitor complex". Biochim. Biophys. Acta 802 :99-110, 1984.
Young, R. A. et al. "Efficient isolation of genes by using antibody probes." Proc. Natl. Acad. Sci. USA, 80: 1194-98, 1983.
O'Dell, W.D., et al. Principles of Competitive Protein Binding Assays. J. B. Lippincott; 1971, pp. 243-254.
Nielsen L.S. et al. "Monoclonal antibodies to human 54,000 Molecular Weight Plasminogen Activator Inhibition from Fibrosarcoma cells--Inhibitor Neutralization and One-Step Affinity Purification," Thrombosis and Haemostasis 55(2) 206-212, 1986.

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