Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – In aerosol – fine spray – effervescent – pressurized fluid,...
Reexamination Certificate
2011-08-23
2011-08-23
Cheu, Jacob (Department: 1641)
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
In aerosol, fine spray, effervescent, pressurized fluid,...
C435S007100, C435S007200
Reexamination Certificate
active
08003075
ABSTRACT:
The present invention includes a diagnostic assay for the detection and determination of MGP in a human serum sample, which comprises the use of one or more antibodies, preferably monoclonal antibodies, specifically recognizing epitopes on and/or conformations of human Matrix Gla-Protein. A method is provided for using MGP-related antigens as biomarkers for certain diseases, for example, atherosclerosis and other vascular diseases, and angiogenesis
eogenesis in tumor development. Monoclonal antibodies of class IgG are described for use in the assay, which are defined herein as mAb3-15, mAb35-49[Glu], mAb35-49[Gla], mAb35-53[Glu], and mAb35-53[Gla]. Polyclonal antibodies and methods are also disclosed for measuring MGP in a human serum sample.
REFERENCES:
patent: 5476779 (1995-12-01), Chen et al.
patent: WO 01/02963 (2001-01-01), None
patent: WO 2005/046455 (2005-05-01), None
Teo et al. American J. of Neuroadiology 1997, vol. 18, p. 576-579.
Harlow et al. “Antibodies a Laboratory Manual,” Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, 1998, p. 141-155.
Schurgers et al. Z Kardiol. 2001 Suppl. 3, p. 111/57.
Shanahan et al. J. Clin. Invest. 1994 vol. 93, p. 2393-2402.
Search Report, European Patent Office, for EP 0 05 07 5994, dated Sep. 29, 2005 (4 pages).
Rutsch, F. and Terkeltaub, M.D. (2003) Parallels bweteen arterial and cartilage calcification: what understandign artery calicification can teach us about chondrocalcosis Current Opinion in Rheumatology 15:302-310.
Spronk, H.M.H., Soute, B.A.M., Schurgers. L.J., Cleutjens, J.P.M., Thijssen, H.H.W., Demey, J.G.R., and Vermeer, C. (2001) Matrix Gla Protein Accumulates at the Border of Regions of Calicification and Normal Tissue in the Media of the Arterial Vessel Wall, Biochem. Biophys. Res. Commun. 298:485-490.
Price et al. (1983) Matrix GLA Protein, A New ?-Carboxyglutamic Acid-Containing Protein Which is Associated with the Organic Matrix of Bone, Biochemical and Biophysical Research Communications 117:765-771.
Otawara et al. (1986) Developmental Appearance of Matrix GLA Protein During Cicification in the Rat, J. Biol. Chem. 261:10828-32.
Fraser et al. (1988) Lung, Heart, and Kidney Express High Levels of mRNA for the Vitamin K-dependent Matrix Gla Protein, Journal of Biological Chemistry 263:11033-11036.
Hauschka et al. (1989) Osteocalcin and Matrix Gla Protein: Vitamin K-Dependent Proteins in Bone, Physiological Reviews vol. 69 No. 3:990-1047.
Shearer, M.J. (1990) Annotation, British Journal of Haematology 75:156-162.
Vermeer, C. (1990) ?-Carboxyglutamate-Containing Proteins and the Vitamin K-Dependent Carboxylase, Biochem. J. 266:625-636.
Sadowski et al. (1991) Production of Polyclonal Antibodies to a Vitamin K-Dependent Epitope of Bone Matrix GLA Protein (MGP) by Synthetic Peptides, FASEB Journal 5:A944.
Hale et al. (1991) Carboxyl-Terminal Proteolytic Processing of Matrix Gla Protein, Journal of Biological Chemistry 266:21145-21149.
Loeser et al. (1992) Artluclar-Cartilage Matrix ?-Carboxyglutamic Acid-Containing Protein, Biochem. J. 282:1-6.
Luo et al. (1997) Spontaneous Calcification of Arteries and Cartilage in Mice Lacking Matrix GLA Protein, Nature 386:78-81.
Proudfoot et al. (1988) Vascular Calcification: New Insights Into an Old Problem, Journal of Pathology 185:1-3.
Proudfoot et al. (1998) Calsification of Human Vascular Cells In Vitro is correlated with High Levels of Matrix GLA Protein and Low Levels of Osteopontin Expression, Arterioscler Trhomb. Vasc. Biol. 18:379-388.
Shanahan et al. (1998) The Role of Gla Proteins in Vascular Calcification, Critical Reviews in Eukaryotic Gene Expression 8 (3&4):357-375.
Price et al, (1998) Warfarin Causes Rapid Calcification of the Elastic Lamellae in Rat Arteries and Heart Valves, Arteriosclerosis Thrombosis and Vascular Biology, 18:1400-1407.
Dhore et al. (1999) Osteocalcin and Matrix GLA Protein Deposition is Enhanced in Human Atherosclerotic Plaques, FASEB Journal, 13:A204.
Braam et al. (2000) Assay for Human Matrix GLA Protein In Serium: Potential Applications In the Cardiovascular Field, Arteriosclerosis Thrombosis and Vascular Biology, 20:1257-61.
Campbell Alisa M. (section 1.3.4. p. 29; Monoclonal Antibody Technology (1984) Elsevier Science Publishers).
Kiefer et al. (1988) The cDNA and derived amino acid sequence for human and bovine matrix Gla protein. Nucleic Acids Res. 16: 5213.
Watson et al. Recombinant DNA, second edition (1996) Ch. 12, Transferring Genes into Mammalian Cells, pp. 217-230.
Cheu Jacob
Kalow & Springut LLP
MGP Diagnostics A/S
Schmidt William D.
LandOfFree
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