Diagnostic assay for cancer susceptibility

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091100, C435S091500, C536S023100, C536S023500, C536S024310, C536S024330, C536S025320

Reexamination Certificate

active

07094538

ABSTRACT:
The lifetime probability of a person developing cancer can now be determined based on an allelic variation found in the 3′UTR of the prohibitin gene. The probability is dependent on the sequence of the 3′UTR at position 729, i.e., whether there is a thymine (T) or a cytosine (C) or both at this position. Determining the sequence at the position 729 can be done by any number of standard techniques. Preferably, the sequence is determined by amplifying this region by PCR and subjecting it to an RFLP analysis.

REFERENCES:
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patent: 5776738 (1998-07-01), Dell'Orco et al.
patent: WO 96/05306 (1996-02-01), None
patent: WO 96/40919 (1996-12-01), None
patent: WO 98/20167 (1998-05-01), None
Bentley et al., “Rapid methods for detection of polymorphic markers in genomic DNA,”Methods in Molecular Biology, in:Protocols in Human Molecular Genetics, 9:51-68, c1991.
Dell'Orco et al., “Differential regulation in prhibitin in prostate cancer cells,”Molec. Biol. Cell, 7:24a, 1992.
Jupe et al., “Prohibition antiproliferative activity and lack of heterozygosity in immortalized cell lines,”Exp. Cell Res., 218:577-580, 1995.
Jupe et al., “Prohibitin in breast cancer cell lines: loss of antiproliferative activity is linked to 3'untranslated region mutations,”Cell Growth and Differentiation, 7:871-878, 1996.
Jupe et al., “The 3'untranslated region of prohibitin and cellular immortalization,”Exp. Cell Res., 224:128-135, 1996.
Liu et al., “Restriction endonuclease fingerprinting (REF): a sensitive method for screening mutations in long, contiguous segements of DNA,”BioTechniques, 18:470-477, 1995.
Sato et al., “The human prohibitin gene located on chromosome 17q21 is mutated in sporadic breast cancer,”Cancer Res., 52:1643-1646, 1992.

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